Part:BBa_K277029

3L.3_23.B1.03

3L.3_23.B1.03 is 756 bases long and is cloned into the pGem-T vector.

3L.3_23.B1.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.03 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

ARS ARS304 (-246..211) Autonomously Replicating Sequence on Chromosome III

Sequence (the first 756 bases correspond to coordinates 20650..21405 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 567
  Illegal PstI site found at 425
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 425
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 567
  Illegal PstI site found at 425
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 567
  Illegal PstI site found at 425
  
• 1000
  COMPATIBLE WITH RFC[1000]